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Description
mIHC Dye (TSA700)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 700, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.3 ★★★★★
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Product Reviews
★★★★★ 4
If you liked 1491, you'll like 1493
Format: Hardcover
I originally read the first edition of 1491, which I loved. So that's why I ordered 1493. At about the same time 1493 arrived, I found out there was a new, revised version of 1491, which my husband bought from another source. So I re-read it at the same time I read 1493 for the first time. The reason I mention this is that there are some similarities between the revised version of 1491 and the newer book, 1493 - actually some repeated material. That's ok, as the author is taking the premise of 1491 another step further. Essentially, 1491 focuses on what new studies show was really going on in the Western Hemisphere before Columbus' arrival, where native peoples were far more numerous and had more advanced cultures than Europeans previously thought possible. In 1493, Charles Mann shows not only how Columbus and Europeans changed the New World, but how the "Columbian exchange" wrought great changes in the other direction as well. And he pulls in the further exchanges with Asia, to show the trans-global linkages of the phenomenon. So, some of his exposition gets a little repetitious, but overall he's an engaging writer, and for those of us who love the history of cultural exchanges and first contact, these books are mandatory reading!
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Reviewed in the United States on October 5, 2011
★★★★★ 5
Truly worth every penny. DS2r?
Format: Hardcover
Truly incredible documentation of the thoughtful work of a handful of artists. I'm hoping that by supporting this book we may inch ourselves closer to a Dead Space 2 remake lead by Motive studio. This book is a must for fans or the game and horror in general. Well made, good quality images, lore drops, developer letters. Its fantastic!
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Reviewed in the United States on May 15, 2025
★★★★★ 5
Nice art
Format: Hardcover
The art is good and I love the comparisons to the og
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Reviewed in the United States on October 12, 2025
★★★★★ 5
Great Art Book
Format: Hardcover, Format: Hardcover
One of my all time favorite games, this was perfect. It IS an art book, so more into the visuals then descriptions or thoughts on designs, but that's about what I expected. Especially as it's more for the remake than original. But still worth every cent, great to see the details (especially things that are tough to see in-game) and small descriptions. Arrived quicky and in perfect condition, no complaints at all!
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Reviewed in the United States on July 16, 2025
★★★★★ 5
Love it.
Format: Hardcover
Art book is amazing!
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Reviewed in the United States on January 29, 2026