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Description
Mouse Lung Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A added. |
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| Description |
Composition:
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| Storage Temp. | 4° C. 3months-20° C. 1 year, see label for details |
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4.4 ★★★★★
Based on 13 reviews
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Product Reviews
★★★★★ 4
Power Adapter Not Included
Color: Brown +Wireless
I'm initially impressed with this valet tray. I purchased two for my husband: one for his nightstand and one for his dresser, as he likes to keep things organized. We liked the idea of the wireless charging capabilities, but were a little disappointed to discover that the power adapter (wall plug) is not included with the tray. The tray includes a charging pad and a cord, but no wall plug. We don't have an extra pair in the house, so we'll have to wait until a pack arrives before we can test out the charging capabilities. That aside, the trays are a handsome set. I think 20% larger might serve us a tad better, but this will do very nicely for now. We're able to fit almost everything we need in the tray and that will leave the tables looking much tidier, even if one or two items don't quite make it in.
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Reviewed in the United States on October 20, 2025
★★★★★ 5
Great organization and a charger too!
Color: Blue
Pros: charge pad works well to charge not only phones but watches as well,materials are quality and the blue color is very appealing. Fits well on the night stand and has great sized compartments for items to stay organized.
Cons: none that I can note
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Reviewed in the United States on October 10, 2025
★★★★★ 5
3 year anniversary is leather
Color: Brown +Wireless
I bought this for our third year wedding anniversary, as a gift for my husband. The 3rd year is supposed to be a gift inspired by leather. The tray is very durable and has lots of compartments. My husband uses it on his nightstand to keep things that he has in his pocket when he empties them the end of the night. The wireless charger is very convenient, although he prefers to actually plug in his phone rather than wirelessly charge it. The charger cable itself is surprisingly of great quality. The leather isn’t too bad either. Good gift for the price point.
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Reviewed in the United States on July 29, 2025
★★★★★ 5
Organizer tray
Color: Brown +Wireless
This thing's style is 1960's cool.. Great organizer and charger.
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Reviewed in the United States on January 24, 2026
★★★★★ 5
Cute Tray, Bad Charger
Color: Black
Worked for about a month, now it will only charge sometimes.
Edit: Seller contacted me and replaced item. It is working perfectly fine now.
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Reviewed in the United States on March 28, 2025