SKU: 78276481238

Human/Mouse/Rat TGF-β1 ELISA Kit

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Description

Human/Mouse/Rat TGF-β1 ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human mouse rat TGF & beta; 1 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The tgf

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human / mouse / rat TGF - & beta; 1 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The tgf-&beta in the sample; 1 bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and TGF - & beta in the sample; The concentration of 1 was positively correlated. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, tgf-β 1 a copy of detection antibody, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, HCl, NaOH, plate sealing membrane and instructions.

Sensitivity: 3.36 pg/ml

Detection Range: 31.3-2000pg/ml

Recovery Range: 83-117%

Storage Method: 2-8 ℃

Standard Curve:

 

 

Background:

Transforming growth factor beta; 1 (tgf-β 1)Is transforming growth factor beta; Polypeptide members of the superfamily have a variety of cytological functions, including the regulation of cell growth, proliferation, differentiation and apoptosis. Tgf-β Cooperate with TGFA to induce transformation. It can also act as a negative autocrine growth factor. Tgf-β Dysregulation of activation and signaling may cause apoptosis. Many cells can synthesize tgf-β, And almost all have specific receptors. Tgf-β 1. Tgf-β 2 and tgf-β 3 all function through the same receptor signaling pathway. Tgf-β It plays an important role in regulating the immune system and has different activities on different types of cells or cells at different developmental stages. Most immune cells (or leukocytes) secrete tgf-β 1. Tgf-β 1 is associated with cancer, autoimmune disease, liver disease, kidney disease, diabetes, cardiovascular disease, asthma, chronic obstructive pulmonary disease (COPD) and cystic fibrosis (CF).

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SKU: 78276481238

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MaryBeth K
Boise, US
★★★★★ 5
Fae Courts with High Intrigue
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This book is one that just builds and builds and then surprises you to no end. You may think you know the villains and then you are jolted in another direction. Princess Reyna is a real gem, strong of character, a fierce fighter, and loyal to her family and kingdom. Just when you think she and Lorcan, well you know, the plot is flipped. Can't wait to see where this goes in book two.
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Reviewed in the United States on July 7, 2023
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Kindle Customer Maureen
Natrona Heights, US
★★★★★ 4
Slow, sorry but good
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This was a slow moving book. Lots of character pov chapters, lots of superfluous descriptions and endless courtly appearances stalled this book to start. Once you get into the heart of the story, it takes off. Before you know it the book is done. My favorite character is Reyna. She is so strong. She is true to herself. She gets into a lot of trouble with her headstrong ways but it's entertaining. I have high hopes for Lorcan. He is honorable to a fault. Thane had turned out to be better than I thought but i still don't like him. Eislin is useless. Great plot twists at the end. I'm looking forward too book 2.
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Reviewed in the United States on June 6, 2020
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Faifre6
Louisville, US
★★★★★ 5
Very detailed! Beautiful world building! Strong Heroine!
Format: Kindle
Starts off a little slow and confusing with different POV’s, but starts to all come together towards the middle to make an elaborate plot line and makes it all worth it. Beautiful world building and attention to detail as well as great writing. The cliffhanger was gut wrenching! Can’t wait for the next book!
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Reviewed in the United States on March 4, 2020
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Isabelle
Carnegie, US
★★★★★ 4
Interesting
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This was a very captivating book once you got into it thoroughly. But the third person perspective was a bit hard to get used to. But as you got into it and followed the different characters, it was interesting and filled with intrigue, conflict and forbidden love. I can’t wait to read the next one and to complete the series.
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Julie R.
Birmingham, US
★★★★★ 5
Great start
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I loved this book! It's funny but still deals with tough themes, like chronic illness, a serial killer on the loose, and a dash of self-harm. The guys are interesting and distinct, we don't know too much about them yet. It does end on a really terrible cliffhanger but on the bright side the next book is out and I believe the series is complete. I have enjoyed both of these authors separately and this is a great team up!
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Reviewed in the United States on May 2, 2025

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