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Description
Mouse SIRT3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Sirtuin 3 (SIRT3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Sirtuin 3 (SIRT3) in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Sirtuin 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Sirtuin 3 (SIRT3), also known as the mitochondrial NAD-dependent deacetylase sirtuin-3, is a protein encoded by the SIRT3 gene. It is a member of the sirtuin protein family and is homologous to the yeast Sir2 protein. It exhibits NAD+-dependent deacetylase activity. Members of the sirtuin family are characterized by a sirtuin core domain and are divided into four classes. The protein encoded by this gene is included in class I of the sirtuin family. Sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance, and metabolic regulation. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.5 ★★★★★
Based on 719 reviews
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Product Reviews
★★★★★ 5
Excellent mouse and more!
Size: One Size
The Logitech M510 wireless mouse is a very comfortable and smoothly performing mouse. It is a full size mouse that nicely fills up the palm of the hand. The weight of the mouse is "just right". The mouse body is symmetrical so that will be equally comfortable for left or right handed people.
The mouse movement and scroll wheel work perfectly smoothly with no jump or jitter. The mouse optical performance is very robust on poor surfaces. It works equally well on a mousepad, a bare table, on a pants leg, back of a book, even the palm of the hand. It tracks perfectly on almost any surface (which many other mouse brands have trouble with).
These mice use the Logitech "Unifying" USB mini receiver which can support up to six Logitech devices by itself. If you have a Logitech mouse, keyboard, trackball, etc... they can all operate from the same receiver (saves using up USB ports). Be aware that the unifying receiver ONLY works with Logitech products and that it is NOT Bluetooth or Wi-Fi. Logitech products can be "paired" with the unifying receiver in Windows and MAC (of course) and also in Linux by using the software package called "solaar" (free, open source). The mouse comes pre-paired to the receiver, so it works "out of the box". If you want to add (up to 5 more devices), you have to pair them yourself.
The battery life is awesome. Because I hate leaking batteries, I always use AA lithium cells for everything (including the mouse). My first old Logitech MS-510 mouse that I purchased over three years ago is still working fine on the FIRST batteries that I installed in it (two AA lithium). Since I use it on my desktop the mouse power switch is always left on. It must consume practically no power when not being used.
If you use the mouse with a laptop or need to move it from computer to computer, the little unifying receiver can be stored inside the mouse (in a little USB sized slot next to the batteries) to avoid losing it.
What follows is some technical information that you can skip if you don't care.
Construction: This mouse is very nicely engineered and can be completely disassembled by removing two little screws inside the battery compartment. No screws hide under the teflon slider pads, so those don't get damaged if you take the mouse apart. Every component then can be removed simply by sliding or snapping it out. Why take it apart? See next.
My personal feeling is that the left and right mouse buttons are too light (i.e. press too easily). The buttons are no lighter than other mice, I just prefer them a bit tighter. To change this, I replace the two little microswitches with Omron Series D2F-01 parts that have a 150 gf actuation force. It takes about twice the pressure to click these switches as compared to the originals. To replace them is super simple: Just unsolder the originals and solder the new ones in place. If you forget which way they go in, the orientation is printed on the PC board.
The side "scroll" buttons already use heavier pressure microswitches, so I don't change these. The "middle button" (pressing the scroll wheel) and the left/right scroll switches (rock the scroll wheel left or right) are a different type of switch that also do not need changing.
If you disassemble the mouse, be aware that the little plastic slider for the power switch will be free to fall out when the mouse PC board is removed. If you don't know this, it's possible for the slider to silently fall out, never to be seen again.
I won't tell you HOW to unsolder and resolder the button microswitches, because if you don't know how, you shouldn't be doing jt in the first place (or get an electronics-knowledgable friend to help). And, of course opening and modifying the mouse will void the warranty!
I will give you one important hint: Because the mouse was assembled using RoHS compliant lead free solder, the microswitch solder joints should first be RE-SOLDERED with leaded solder before sucking or wicking the joints clean. This is done to "alloy" the high temperature lead free solder into more manageable leaded solder to avoid burning/ruining the (single sided) PC board.
Good luck! This mouse (hacked or not) gets 5 stars and both thumbs up. It works flawlessly, it's comfortable, battery life is amazing and the price is good (even better when it's sometimes on sale). Grab one!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 27, 2020
★★★★★ 5
Excellent mouse, good size, enough buttons
Size: One Size
Easy to setup. Fit hand well. Has enough buttons to do everything I want. Good price.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 5
10/10 work mouse
Size: One Size
Lasts forever. Been my work mouse for years. Batteries last forever in it. Cheap enough to keep a spare in my laptop bag for when I need to travel for work. Not a gaming mouse, but a serviceable work mouse. But not tiny like a "travel mouse" so it isn't uncomfortable to use. A man can use one without his hand cramping up.
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Comfortable Seating
Color: Black, Style: Classic
I honestly didn’t expect this chair to be this comfortable, but it has exceeded my expectations in every way. I sit at my desk for long hours every day, and the adjustable air-pump lumbar support has been a total game changer. I love that I can fine-tune the firmness depending on how my back feels that day. My lower back pain has noticeably improved since switching to this chair.The seat is wide, thick, and very supportive without feeling stiff, which makes long work sessions much more comfortable. The armrests are another big plus—they adjust in so many directions that I can always find the perfect position for typing, reading, or relaxing. The chair feels extremely sturdy and well built, and it gives me confidence knowing it supports higher weight without wobbling.Assembly was straightforward and took about 20 minutes. Overall, this chair feels premium, comfortable, and thoughtfully designed. I would absolutely recommend it to anyone looking for a reliable, all-day office chair.
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Reviewed in the United States on March 28, 2026
★★★★★ 5
Nice chair, w/ good cust support
Color: Black, Style: Classic
I apologize for length, but if I am doing a review for other people I assume you want to have as
much info as possible to make a decision. I feel this chair is for tall/fat/both so will review it as
such. At 6’4” @ 350lbs I fit into both, but reviewing based upon my body, yours is different so
adjust according.
TLDR – Would buy again, cavernous comfy chair with excellent customer support. Only
personal dings against chair are the back could be taller, but added back due to support.
Parts + Fit/Finish + Assembly:
The parts have some heft and feel solid as expected for a chair designed for heavy people.
Material and stitching look proper and I see no flaws. My box was beat to hell and ripped
opened at one point. Packing material took the hit and did its job as the items were unaffected.
The gas cylinder for the chair was missing and assume lost during shipping. (see customer
service). Assembly was standard office chair fare with simple and clear instructions.
Wheels / Height / Controls:
I normally wouldn’t think of mentioning wheels, but it’s different than expected. They are
good, maybe too good. A slight kick to push you back on wood floors sends you flying. The
height adjustment for me is good/standard. At full height my feet are flat on the floor and
where I would have the chair even if it went higher. My wife (5’6”) at that height is about 2.5
inches above the floor. Taller people than myself may wish for more height. Chair controls are
standard fare and operate smoothly
Seat:
It’s large and with the arms is there is nothing even close to your hip/legs. I can put my fist on
my hips and my arms barely touch the chair arms. This is why you are thinking of this chair, and
for that you will not be disappointed. The seat is not pillow soft and IMHO a little firmer than I
liked at first, but after a few days got broken in or I adjusted. I am a programmer + gamer so
spend a lot of time in the chair for hours on end. Comfortable and no complaints at all. Seat
depth for me is ~1” from my knee and good with roughly 20.5” usable butt/thigh length. That
should be fine for big/tall but may be a bit much for big/short. Measure your current chair and
decide from there.
Back:
The back lets the chair down a bit for me, the big/tall user, as the target seems to be big/short
users. The back ends just below my shoulder-line. That obviously means no leaning my head
back and the headrest is squarely on my shoulder blades. Not uncomfortable, but I’d prefer a
taller back. For me personally I’d like it to be extended 4”, but that would likely be an issue for
shorter users. I feel if the manufacturer increased the back by 2 inches it would be an ideal
compromise for all users. The chair has an inflatable lumbar support, which I did not use as I
personally find those uncomfortable. Also note the chair is designed for a more proper posture
with a 90deg back and not a more relaxed 100-110deg. Neither pro/con as personal
preference.
Arms:
The design of the arms being supported by rear only as mentioned earlier allow for an
abundance of hip/thigh space. This is an excellent ergonomic design with a potential caveat.
More stress will be placed upon the connection/joint. The joint for this chair is just shy of 4” so
seems beefy but only time will tell if it holds up to the lateral force of leaning on it while
reading. My chairs tend to last 5-7 years, due to the right arm feeling wobbly or the chair
leaning to the right. The arm adjustments provide a nice front/back of ~3”, from touching chair
back to 3” gap between chair/armrest. The height is similar and also moves the armrest
forward about another 2”. At full forward/up the armrest are higher than I like so should easily
accommodate taller users.
Customer Service
My chair was missing a part that I suspect was lost during shipping due to box condition. I
contacted them Sun (7/20/2025 @ 1201). At 2228 that night I received a response that they
acknowledged the issue and gave a tracking order for the missing part. The part arrived Mon
7/28 and gave a few days to test it out for review Fri (8/1). Obviously you don’t want an issue,
but if an issue arises how a company handles it tells you a lot IMHO on how they feel about
their product. I am very pleased with their handling of the matter.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 4, 2025
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