SKU: 84930630449

Human IL-1β High Sensitivity ELISA Kit

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Description

Human IL-1β High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human il 1 Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il 1&beta in the sample; Binding to solid phase antibodies.

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human il-1β Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il-1&beta in the sample; Binding to solid-phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and il-1&beta in samples; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, il-1β One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.02pg/ml

Detection Range: 0.19-12 pg/ml

Recovery Range: 93-112%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 1 (IL-1) is a 17kDa extracellular polypeptide, which is divided into il-1α and il-1β two proteins. Il-1β is a protein precursor secreted by activated macrophages, which is secreted by caspase1 (casp1/ice)The activated form produced by hydrolysis. This cytokine is an important mediator of the inflammatory response and is involved in many cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 is an important family of proteins, which are derived from bioactive monocytes and participate in inflammatory and immune responses. Il-1β Elevated expression can produce a variety of different autoinflammatory syndromes, the most significant of which is the monogenic condition known as caps, which is due to the triggering of il-1β It is caused by mutations in the processed inflammasome receptor NLRP3. This kit is used to detect human il-1β Of the mature form and the uncleaved precursor form.

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SKU: 84930630449

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4.8 ★★★★★
Based on 6 reviews
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Megan
Chelsea, US
★★★★★ 4
Seems to work for damaged barrier (accidental damage as well as eczema and rosacea).
Size: 6.76 Ounce (Pack of 1)
SO I am currently tryign to repair a damaged skin barrier. I have both rosacea and eczema so it's nearly impossible to find a product that makes both conditions happy. I started out using the cream version of this but my face always felt dry with it. I decided to try this since a balm usually helps keep in moisture much better. I am only using a gentle cleanser and this, planning on adding in a toner at some point. At first I thought I was sent the cream again because this comes out like their cream version but as you rub it to spread, you can tell it has a thicker feel to it. It spreads easily and doesn't tug at my skin. Seems to sink in pretty quickly but it does leave a sticky film until it completely absorbs. I can feel my eyelids stick while waiting. Not sure how long it takes to fully absorb because once it does on my eyelids, I forget. But the stickiness does go away and leaves a soft texture behind. I do find some itching in places I normally get when my skin dislikes an ingredient. But I'm not sure if it's my normal or current damaged barrier. It helps with the dry feeling and I would say I only feel a slight tightness. But so far, the best moisturizer I have used. And I love that there is NO NIACINAMIDE, NO HYALURONIC ACID, no oat, no shea butter, and no cica/centella asiatica. These ingredients wreak havoc on my skin and everybody is adding this to everything. It's been so hard to find a moisturizer.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 18, 2025
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Verified Purchase
GAIL SUTHERLAND
Grantham, US
★★★★★ 5
Works quickly to calm itch
Size: 6.76 Ounce (Pack of 1)
I had an itchy outbreak on the back of my hand the day this product arrived. Perfect timing. Applied Avene and within a short time the itch calmed almost 100%. Slept through the night and this morning, the red rash is about 95% healed. So glad I bought this. Just another old age issue to deal with.
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Reviewed in the United States on May 21, 2026
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sandraday
Houston, US
★★★★★ 5
Not greasy and no fragrance
Style: Updated Formula
Excellent moisturizer for my sensitive skin.
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Reviewed in the United States on May 26, 2026
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Alice Cooper
Houston, US
★★★★★ 5
Great lotion for very dry skin
Style: Updated Formula
I had a laser done on my face and the products they gave me weren't working. This came highly recommended so I gave it a try. I'm very glad I did! It moisturized my face really well without appearing greasy. I used the rest when my face got dry from winter (in th midwest). Felt very rich and smooth. Medium thickness.
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Reviewed in the United States on December 19, 2025
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Kcm228
Charlottesville, US
★★★★★ 5
Helps repair skin barrier
Style: Updated Formula
This stuff is amazing! It has drastically helped repair my skin barrier. I had a flare up of dermatitis right before an important event and this helped clear it!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026

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