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Description
Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)Product Specification References 1. Mingzhu Yang, et al., Chemical induced chromatin remodeling reprograms mouse ESCs to totipotent like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle enhanced radiotherapy synergizes with PD L1 blockade to limit post surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and
Product Specification
| References | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. Ed Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Usage | 1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. |
| Experimental Procedure | https://univ.oss-cn-shanghai.aliyuncs.com/absin-china/images/experiment/20190514165940524.png |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Synonym | Multicolor kit |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single index, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 540, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI. |
| General Notes | 1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management. |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC). |
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4.2 ★★★★★
Based on 11 reviews
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Product Reviews
★★★★★ 4
If you liked 1491, you'll like 1493
Format: Hardcover
I originally read the first edition of 1491, which I loved. So that's why I ordered 1493. At about the same time 1493 arrived, I found out there was a new, revised version of 1491, which my husband bought from another source. So I re-read it at the same time I read 1493 for the first time. The reason I mention this is that there are some similarities between the revised version of 1491 and the newer book, 1493 - actually some repeated material. That's ok, as the author is taking the premise of 1491 another step further. Essentially, 1491 focuses on what new studies show was really going on in the Western Hemisphere before Columbus' arrival, where native peoples were far more numerous and had more advanced cultures than Europeans previously thought possible. In 1493, Charles Mann shows not only how Columbus and Europeans changed the New World, but how the "Columbian exchange" wrought great changes in the other direction as well. And he pulls in the further exchanges with Asia, to show the trans-global linkages of the phenomenon. So, some of his exposition gets a little repetitious, but overall he's an engaging writer, and for those of us who love the history of cultural exchanges and first contact, these books are mandatory reading!
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Reviewed in the United States on October 5, 2011
★★★★★ 5
Truly worth every penny. DS2r?
Format: Hardcover
Truly incredible documentation of the thoughtful work of a handful of artists. I'm hoping that by supporting this book we may inch ourselves closer to a Dead Space 2 remake lead by Motive studio. This book is a must for fans or the game and horror in general. Well made, good quality images, lore drops, developer letters. Its fantastic!
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Reviewed in the United States on May 15, 2025
★★★★★ 5
Nice art
Format: Hardcover
The art is good and I love the comparisons to the og
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Reviewed in the United States on October 12, 2025
★★★★★ 5
Great Art Book
Format: Hardcover, Format: Hardcover
One of my all time favorite games, this was perfect. It IS an art book, so more into the visuals then descriptions or thoughts on designs, but that's about what I expected. Especially as it's more for the remake than original. But still worth every cent, great to see the details (especially things that are tough to see in-game) and small descriptions. Arrived quicky and in perfect condition, no complaints at all!
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Reviewed in the United States on July 16, 2025
★★★★★ 5
Love it.
Format: Hardcover
Art book is amazing!
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Reviewed in the United States on January 29, 2026