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Description
people β2 microglobulin ELISA KitProduct Specification Usage Specimen requirements: 1. Extract specimens as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be stored at 20 , but repeated freezing and thawing should be avoided 2. Samples containing NaN3 cannot be detected because NaN3
Product Specification
| Usage | Specimen requirements: 1. Extract specimens as soon as possible after collection, and the extraction should be carried out according to relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be stored at-20 ℃, but repeated freezing and thawing should be avoided 2. Samples containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP). Operation steps: 1. Dilution of standard product: This kit provides one original standard product. Users can dilute it in a small test tube according to the following chart.
2. Sample addition: Set up blank wells (the blank control wells do not add samples and enzyme-labeled reagents, and the other steps are the same), standard wells, and sample wells to be tested respectively. Accurately add 50uL of the standard on the enzyme-labeled coated plate, first add 40uL of sample diluent to the sample well to be tested, and then add 10uL of the sample to be tested (the final dilution of the sample is 5 times). Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well. 3. Incubation: Incubate at 37 °C for 30 minutes after sealing with a plate sealing film. 4. Liquid preparation: 30 times concentrated washing solution is diluted 30 times with distilled water and used for later use 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each hole with washing liquid, let it stand for 30 seconds and then discard it. Repeat this 5 times and pat dry. 6. Enzyme addition: 50uL of enzyme-labeled reagent is added to each well, except for blank wells. 7. Incubation: The procedure is the same as 3. 8. Washing: The operation is the same as 5. 9. Color development: First add 50uL of color developer A to each well, then add 50uL of color developer B, gently shake and mix well, and develop color at 37 °C in the dark for 10 minutes. 10. Termination: Add 50uL of stop solution to each well to terminate the reaction (blue immediately turns yellow at this time). 11. Measurement: Zero the blank well, and measure the absorbance (OD value) of each well sequentially at a wavelength of 450nm. The determination should be performed within 15 minutes after the addition of the stop solution. Calculation: Taking the concentration of the standard substance as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find out the corresponding concentration from the standard curve according to the OD value of the sample. Multiply by the dilution factor. Or use the concentration of the standard substance and the OD value to calculate the linear regression equation of the standard curve, substitute the OD value of the sample into the equation, calculate the sample concentration, and then multiply it by the dilution factor, which is the actual concentration of the sample. |
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| Species Reactivity | Human | ||||||||||||||||||||||||
| Theory | This kit uses double antibody sandwich method to determine the level of human β2-microglobulin in specimens. The microplate is coated with purified human β2-microglobulin antibody to make solid phase antibody, and β2-microglobulin is sequentially added to the microwells coated with monoclonal antibody, and then combined with HRP-labeled β2-microglobulin antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, substrate TMB is added to develop color. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and β2-microglobulin in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of human β2-microglobulin in the sample was calculated by the standard curve. | ||||||||||||||||||||||||
| Synonym | BMG/β2-MG ELISA Kit | ||||||||||||||||||||||||
| Composition | Kit composition:
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| General Notes | 1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag. 2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing. 3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples. 4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating. 5. The sealing film is only for one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious agents. 9. Components of different batch numbers of this reagent shall not be mixed. |
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| Storage Temp. | Store at 2-8 °C, shelf life 6 months. | ||||||||||||||||||||||||
| Test Range | 3ug/L-125ug/L | ||||||||||||||||||||||||
| Applications | It is used to determine the content of β2-microglobulin in human serum, plasma and related fluid samples. |
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4.4 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 5
$100 helping small business 😄
I love this 😍 It will help a small business buy labels, or just something that they need to function and continue their business!
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Reviewed in the United States on May 13, 2026
★★★★★ 4
Interesting Mutter
interesting
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Convenient Accessibility
It's great to have a company card that my employees can have access to for office supplies and other purchases. Thank you Amazon 🙏
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Reviewed in the United States on May 13, 2026
★★★★★ 5
The Christmas movie no TV stations ever seem to show during the holidays
Since Amazon lumps the reviews for all the various releases of this movie together, I want to be clear that this review is for the single disc, 132 minute blu-ray version with the cover picture of smiling man made of bricks with the word "Brazil" flying out of the top of his head.
For those who don't know the history of this movie - there are at least three different versions. It was originally released in Europe as a nearly two and half hour long film. The studio who financed it didn't like that version because it A. was too long, B. was too uncommercial and C. had a dark, depressing ending. The contract they had with Gilliam stipulated that the movie had to be less than 2 hours and 15 minutes, so it could have multiple prime-time showings in theaters. They suggested he cut the ending.
Gilliam refused, so the studio took it upon themselves to create the "Love Conquers All" version, which chopped out almost an hour of footage - they only kept the parts that showed the main character Sam in a heroic light, anything that contributed to the Sam/Jill love story and anything that featured Robert DiNero. And, of course, the ending was changed to a happy ending by removing the final scene. Also, to make the plot easier to follow, they used some alternate takes, some deleted footage and hired voice actors to overdub dialog.
Unsurprisingly, Gilliam didn't want that version released, so he finally edited the movie down to 2 hours and 12 minutes, kept the dark ending and made a few other minor changes and the studio was contractually obligated to release that version to theaters in the United States.
It's this US version that appears on the blu-ray, with absolutely no additional features (not even the Gilliam commentary that's included on most other releases of the film). Annoyingly, they also added advertisements for other movies to the main menu screen. But I'm still happy to have this version, because it's the first time I've seen the "US edit" since originally seeing the film in a theater on the campus of Penn State back in my college days.
I can't really write an unbiased review of the movie itself, because from that first viewing I became obsessed with both Brazil and Terry Gilliam's films in general. I bought all his movies on VHS as soon as they came out and eventually replaced them with DVDs. I bought the 3-DVD Criterion "Final Cut" version of Brazil back when it was an expensive new release. I was surprised that it included scenes I'd never seen before - I think it's based mostly on the European version, with some additional tweaks by Gilliam. It also included the "Love Conquers All" version of the movie and a ton of bonus features. Sadly though, it wasn't animorphic, so on my widescreen TV it put black letterboxing bars on all four sides, shrinking the picture down to about 60% of the screen.
This blu-ray was exactly what I was looking for. While it's missing a few (non-essential) scenes, it's the version of the movie that I "grew up" with and is presented in full 16X9 animorphic widescreen. It's the best looking version I've seen since originally seeing it in the theater. For example, I never really noticed the makeup on Katherine Hellmond at the beginning of the movie to make her look older (pre-surgery), but it's clearly visible on the blu-ray. I've always felt bad for one actress during the explosion scene in the restaurant because it looks like she really does get hit by some of the flying debris - the blu-ray is maybe a little too clear there, because now I can tell it's just a dressed-up mannequin.
As for the movie itself - it's one of those films you'll either love or hate. Its alternate-reality, retro-futuristic look makes it seem nearly as fresh and amazing today as it did when it first came out. And the background plot of a government bureaucracy turning a country into a surveillance state in response to a perceived terrorist threat - I know it was based on things happening in England at the time, but it's like Gilliam had a crystal ball and looked 30 years into the United States' future. The alternating between "reality" and dream sequences and the general Gilliam quirkiness will probably put a lot of people off, but it's what drew me to this movie in the first place.
If you become fanatical about the movie like I am, you'll need both this blu-ray and the Criterion release. To fully experience the film you need to see every version (even the Love Conquers All version) because each one includes bits that aren't in any of the others.
If you do decide to watch Brazil and find that you like it, give some of Gilliam's more recent movies a try, like The Imaginarium of Dr. Parnassis and The Zero Theorem. Really, if you like odd, out-of-the-mainstream movies that you'll be thinking about for days after you see them, you can't go wrong with pretty much any Terry Gilliam film.
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Reviewed in the United States on January 11, 2016
★★★★★ 5
One Of My Top Ten Movies
It's a Terry Gilliam masterpiece that blends a touch of Monty Python, Twilight Zone, and Broadway! Worth the watch!
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Reviewed in the United States on May 3, 2026