SKU: 44867423531

Mouse IL-10 ELISA Kit

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Description

Mouse IL-10 ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich enzyme linked immunosorbent assay technology. The specific anti mouse il 10antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il 10present in the sample combines with the solid phase antibody and detection antibody to

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse il-10antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il-10present in the sample combines with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin (streptavidin HRP) was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nmwavelength (reference correction wavelength 540nmor 570nm)

Detection Type: double antibody sandwich method

Form: pre coated 96orifice plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading amount: 100ul

Kit Components:

Pre coated 96well plates, standards, anti mouse il-10detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrpA copy of sealing film and instructions.

Sensitivity: 1.8 pg/ml

Detection Range: 31.2 - 2000 pg/ml

Recovery Range: 95-103%

Storage Method: 2-8℃

Background:

Mouse interleukin -10 (IL-10) also known as cytokine synthesis inhibitor (CSIF). It is IL-10 & Alpha-a member of the helical cytokine family, which also includes il-19, il-12, il-22, IL-24 , and il-26/ak155. Many activated hematopoietic stem cells, hepatic stellate cells, keratinocytes, and placental cytotrophoblasts secrete il-10. Human IL-10 is active in mouse cells, but mouse IL-10 does not act on human cells. The amino acid homology of mature mouse IL-10 and rat IL-10 is 85%, and that of cattle, dogs, horses, cats, humans, sheep, and pigs is 70~77%. Mature mouse IL-10 is a noncovalently bound homodimer with a molecular weight of 36 kDa containing two intrachain disulfide bonds.

IL-10 by the II type cytokine receptor IL-10 r& Alphaand IL-10 & amp; Betathe heteromeric receptor complex that is composed of exerts its biological activityIL-10 r& Alphais a 110 kDa transmembrane glycoprotein expressed in lymphocytes, natural killer cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells, while 75 kDa il-10r& Beta

IL-10 is involved in immune regulation of inhibitory and activating effectsIL-10 as an anti-inflammatory cytokine, promotes the formation of M2 macrophages by inhibiting the activation and expansion of Th1 cells and Th17 cells. Immunosuppression regulates the expression of T cells and B cells, which play an important role in Treg cell proliferation (19) . In the tumor microenvironment, IL-10 inhibition regulates the proliferation of T cells and myeloid derived suppressor cellsIL-10 induces the accumulation of intratumoral material and activates cd8+ T cellsIL-10 plays an important protective role in limiting tissue damage in joint inflammation and promoting muscle regrowth after injury, but also contributes to persistent viral infection. IL-10 is elevated in Sjogren's syndrome (saliva) and primary central nervous system lymphoma.

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SKU: 44867423531

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4.5 ★★★★★
Based on 21 reviews
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A
Verified Purchase
Alan
San Leandro, US
★★★★★ 5
I use it for myself not my pet
I sewed a fluffy velcro pouch to put this in, and then I wrapped the pouch around a microwavable heat gel pack in order to get the sensation of both heat and heartbeat while hugging a large stuffed animal. It works great. It's not too loud if you position it right. It helps me to fall asleep and not feel lonely or touch starved. The battery lasts a long time and I don't have to recharge it very often. I only wish that the speed of the heartbeat was adjustable so that I could slow it down a little more so that my own heartbeat might try to match it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 7, 2024
M
Verified Purchase
Mary S.
Dallas, US
★★★★★ 1
Easily Exposed Lithium Ion Battery
I am returning this item for my money back. Apparently, they think they can charge $16 for a piece of plastic and not even put a screw on the back to hold the panel closed. My dog is smart and a little rough when anxious. I wanted a rechargeable heart (to avoid buying batteries) to help with this anxiety but cannot risk the Velcro coming undone and him biting the heart by mistake. The panel slides off very easily. There's a reason that lithium ion batteries aren't usually easily accessible. I'm not risking my dog discovering a battery and wire in the heart. Even with appropriate supervision, accidents happen. I don't even understand why I would need to access the battery when it's rechargeable. I don't recommend purchasing this product for that reason alone.
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Reviewed in the United States on July 17, 2022
M
Verified Purchase
Marilyn
Phoenix, US
★★★★★ 4
Works great!
It works great, don't have to buy batteries. 07/-8/25 Marilyn A.
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Reviewed in the United States on July 9, 2025
S
Verified Purchase
Seaglass
Birmingham, US
★★★★★ 5
bottle baby kittens preferred this heartbeat to having warm snuggle safe heat disk
Ok... when I got this I thought.. "are you kidding me?" they are going to like this. Joke was on me. They love it. It is rhythmic and it is strong enough that it pulsates the area so all of them can feel it. They consistently would lay on top of the heart rather than in the area of their "crib" where the heat was.. and this was when they were still only a few days old! It works. So this is what you want to know. It beats continuously for about 4-5 days without needing a recharge. Recharge is fast and only takes a couple hours The button is slightly "inside" the unit so when it is placed upside down there is no way that it can accidentally be turned off. Which is great. If the button were on the upside in the pet space then a foot or body part of the little animal could turn it off inadvertantly. Design to put the button slightly inset is extremely important design feature - makes this excellent. When I turn it on I know it will be going as long as the battery still has charge. .   I don't think it is waterproof so I put it under the pee pad that I had under the blanket bedding and it was still easily heard AND felt. If not using a pee pad I would definitely put it in a small ziplock bag to protect from any leaked urine or liquids to keep it safe. When the kittens were done eating they would gravitate towards the heart beat... (with their eyes open or when they were still closed after birth it was the same response). This little gadget was a total lifesaver as it really calmed the kittens in ways I would not be able to. These pictures are of the trio that LOVED this.... Meet Zen (tabby M with white feet), Sugar (tuxedo F with white feet) and Rocket (ginger - M). All cisgender. they were about 3 days old when I got them to foster.. photos from arrival until about 3 weeks of age. Photo with q-tip is to give relative size on arrival. They will be up for adoption in Tustin CA in another 2-3 weeks time.
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Reviewed in the United States on September 17, 2023
L
Verified Purchase
Little Brown Dog
Natrona Heights, US
★★★★★ 5
Fantastic!
The heart that came with Snuggle Puppy was a pain to replace the battery. This is the only rechargeable heart I could find searching online. I was a little concerned reading it’s a “frequently returned item.” But happy to say, we will not be returning this! It shipped charged and the heart is still beating non-stop days later! It’s sturdy and fits in the snuggle puppy perfectly. We have a tiny 17 yr old Chihuahua who never chewed anything up so it’s completely safe for her to enjoy. It helps with her anxiety. You can hear the beat in the video—it’s nice and loud and has a bit of a vibration. Our pup has been sleeping next to it every night. Very happy with this purchase. From reading reviews, I trust if we have an issue with it, their customer service will help. For now, it works! I really like that it has time options—can set it for 2 hrs of heart beating or non-stop.
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Reviewed in the United States on September 17, 2024

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