SKU: 50423812708

Human IL-12/IL-23 p40 ELISA Kit

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Description

Human IL-12/IL-23 p40 ELISA KitProduct Specification Species Reactivity Human Synonym Human IL 12 ELISA kit, IL 23 p19 IL 12 p40, IL23, IL 23, IL 23A, IL 23 A, IL 23p19, IL 23p19 IL 12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin 23 subunit alpha, Interleukin 23 subunit p19, JKA3 induced upon T cell activation, MGC79388, SGRF, SGRFIL 23 subunit alpha, Description Detection principle: Double antibody sandwich ELISA was used in this

Product Specification

Species Reactivity Human
Synonym Human IL-12 ELISA kit, IL-23 p19/IL-12 p40, IL23, IL-23, IL-23A, IL-23-A, IL-23p19, IL-23p19/IL-12p40, IL23P19P19, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T-cell activation, MGC79388, SGRF, SGRFIL-23 subunit alpha,
Description

Detection principle:

Double antibody sandwich ELISA was used in this experiment. Anti-human IL-12/IL-23p40 monoclonal antibody is coated on a microplate, and IL-12/IL-23p40 in samples and standards will bind to the antibody immobilized on the plate, and the free components will be washed away; Horseradish peroxidase-labeled anti-human IL-12/IL-23 p40 polyantibody was added and unbound antibody was washed away; Adding a substrate solution (chromogenic agent), the color of the solution is proportional to the bound target protein; A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm).

Test Type:Double antibody sandwich method

Form:Pre-coated 96-well plate

Test Sample Type:Cell supernatant, serum, plasma

Sample Load:100ul

Kit Components:

A copy of the pre-coated 96-well plate, standard, anti-human IL-12/IL-23 p40 detection antibody, buffer for dilution, chromogenic solution (A, B), wash solution, stop solution, SA-HRP, plate sealing membrane, and instructions.

Sensitivity:3.5 pg/mL

Detection range:62.5-4,000 pg/mL

Recovery Range:89-114%

Save Method:2-8℃

Standard curve plot

Background:

IL-12, also known as natural killer cell stimulating factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine mainly produced by antigen-presenting cells (monocytes/macrophages, dendritic cells, and B lymphocytes). IL-12 has a variety of effects on T lymphocytes and natural killer (NK) cells, including the ability to stimulate cytotoxicity, proliferation, cytokine production, and Th1 subset development.

IL-12 is a 70 kDa (p70) heterodimeric glycoprotein linked by disulfide bonds, consisting of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subbases do not possess IL-12 activity in themselves, but homodimers of p40 have been shown to bind IL-12 receptors and are IL-12 antagonists.

The genes of human p40 and p35 located on chromosomes 5 and 3, respectively, are independently regulated. The expression of p35 mRNA has been found to be almost ubiquitous, however, the p35 subunit was not detected in the culture supernatant of cells expressing p35 or p35 and p40 mRNA only. The p40 mRNA expression level was higher in cells expressing both p35 and p40 mRNA, and together with the heterodimeric IL-12 p70, a free p40 subunit unrelated to the p35 subunit was secreted. Most of the free p40 subunits secreted by the various human cell lines examined have been found to exist in monomeric form. The content of free p40 far exceeds p70 in the supernatants of various activated human monocytes, and the levels of p70 measured by bioassays are consistent with those measured using p70-specific immunoassays, suggesting that p40 monomers are not potent IL-12 antagonists. Currently, the physiological role of the free p40 subunit is unclear.

 

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SKU: 50423812708

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4.1 ★★★★★
Based on 13 reviews
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Verified Purchase
Alan
Fort Morgan, US
★★★★★ 5
I use it for myself not my pet
I sewed a fluffy velcro pouch to put this in, and then I wrapped the pouch around a microwavable heat gel pack in order to get the sensation of both heat and heartbeat while hugging a large stuffed animal. It works great. It's not too loud if you position it right. It helps me to fall asleep and not feel lonely or touch starved. The battery lasts a long time and I don't have to recharge it very often. I only wish that the speed of the heartbeat was adjustable so that I could slow it down a little more so that my own heartbeat might try to match it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 7, 2024
M
Verified Purchase
Mary S.
Chelsea, US
★★★★★ 1
Easily Exposed Lithium Ion Battery
I am returning this item for my money back. Apparently, they think they can charge $16 for a piece of plastic and not even put a screw on the back to hold the panel closed. My dog is smart and a little rough when anxious. I wanted a rechargeable heart (to avoid buying batteries) to help with this anxiety but cannot risk the Velcro coming undone and him biting the heart by mistake. The panel slides off very easily. There's a reason that lithium ion batteries aren't usually easily accessible. I'm not risking my dog discovering a battery and wire in the heart. Even with appropriate supervision, accidents happen. I don't even understand why I would need to access the battery when it's rechargeable. I don't recommend purchasing this product for that reason alone.
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Reviewed in the United States on July 17, 2022
M
Verified Purchase
Marilyn
Waukegan, US
★★★★★ 4
Works great!
It works great, don't have to buy batteries. 07/-8/25 Marilyn A.
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Reviewed in the United States on July 9, 2025
S
Verified Purchase
Seaglass
Houston, US
★★★★★ 5
bottle baby kittens preferred this heartbeat to having warm snuggle safe heat disk
Ok... when I got this I thought.. "are you kidding me?" they are going to like this. Joke was on me. They love it. It is rhythmic and it is strong enough that it pulsates the area so all of them can feel it. They consistently would lay on top of the heart rather than in the area of their "crib" where the heat was.. and this was when they were still only a few days old! It works. So this is what you want to know. It beats continuously for about 4-5 days without needing a recharge. Recharge is fast and only takes a couple hours The button is slightly "inside" the unit so when it is placed upside down there is no way that it can accidentally be turned off. Which is great. If the button were on the upside in the pet space then a foot or body part of the little animal could turn it off inadvertantly. Design to put the button slightly inset is extremely important design feature - makes this excellent. When I turn it on I know it will be going as long as the battery still has charge. .   I don't think it is waterproof so I put it under the pee pad that I had under the blanket bedding and it was still easily heard AND felt. If not using a pee pad I would definitely put it in a small ziplock bag to protect from any leaked urine or liquids to keep it safe. When the kittens were done eating they would gravitate towards the heart beat... (with their eyes open or when they were still closed after birth it was the same response). This little gadget was a total lifesaver as it really calmed the kittens in ways I would not be able to. These pictures are of the trio that LOVED this.... Meet Zen (tabby M with white feet), Sugar (tuxedo F with white feet) and Rocket (ginger - M). All cisgender. they were about 3 days old when I got them to foster.. photos from arrival until about 3 weeks of age. Photo with q-tip is to give relative size on arrival. They will be up for adoption in Tustin CA in another 2-3 weeks time.
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Reviewed in the United States on September 17, 2023
L
Verified Purchase
Little Brown Dog
Carnegie, US
★★★★★ 5
Fantastic!
The heart that came with Snuggle Puppy was a pain to replace the battery. This is the only rechargeable heart I could find searching online. I was a little concerned reading it’s a “frequently returned item.” But happy to say, we will not be returning this! It shipped charged and the heart is still beating non-stop days later! It’s sturdy and fits in the snuggle puppy perfectly. We have a tiny 17 yr old Chihuahua who never chewed anything up so it’s completely safe for her to enjoy. It helps with her anxiety. You can hear the beat in the video—it’s nice and loud and has a bit of a vibration. Our pup has been sleeping next to it every night. Very happy with this purchase. From reading reviews, I trust if we have an issue with it, their customer service will help. For now, it works! I really like that it has time options—can set it for 2 hrs of heart beating or non-stop.
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Reviewed in the United States on September 17, 2024

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