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Description
Human TNF-α ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human TNF antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human TNF-α antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, TNF-α present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, tnfa, tnf-a, TNFalpha, TNF-alpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor, tumor necrosis factor-alpha | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Human TNF-α is a 26kDa type II transmembrane protein consisting of a 35 amino acid (aa) intracellular domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). In the ECD region, human TNF-α has 97% amino acid sequence homology with macaque monkey, and 71-92% amino acid sequence homology with cattle, dog, cotton rat, horse, cat, mouse, pig and rat. It can be expressed by a variety of different cells such as immune cells, epithelial cells, endothelial cells, tumor cells. TNF-α can induce lysis of tumor cells and virus-infected cells, and binds to soluble TNFRI to generate transmembrane transfer signals of downstream cells. TACE/ADAM17 can cause the shedding of TNF-α-containing cell membrane to release active TNF-α cytokine. It is a trimer composed of TNF-α extracellular soluble structure with a molecular weight of 55 kDa. TNF-α has two receptors: TNFRI and TNFRII. TNFRI has a molecular weight of 55-60kDa and is widely expressed; TNFRII has a molecular weight of 78-80 kDa and is limited to hematopoietic cell expression. Both are expressed as homotrimers; TNF-α and TNFRI and TNFRII bind with similar affinities and can promote the activation of NFκB. However, only TNFRI has a cell death domain, which can trigger apoptosis. Both soluble receptors are released into human serum and urine and can neutralize the activity of TNF-α. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.5 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 5
I use it for myself not my pet
I sewed a fluffy velcro pouch to put this in, and then I wrapped the pouch around a microwavable heat gel pack in order to get the sensation of both heat and heartbeat while hugging a large stuffed animal. It works great. It's not too loud if you position it right. It helps me to fall asleep and not feel lonely or touch starved. The battery lasts a long time and I don't have to recharge it very often. I only wish that the speed of the heartbeat was adjustable so that I could slow it down a little more so that my own heartbeat might try to match it.
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Reviewed in the United States on December 7, 2024
★★★★★ 1
Easily Exposed Lithium Ion Battery
I am returning this item for my money back. Apparently, they think they can charge $16 for a piece of plastic and not even put a screw on the back to hold the panel closed. My dog is smart and a little rough when anxious. I wanted a rechargeable heart (to avoid buying batteries) to help with this anxiety but cannot risk the Velcro coming undone and him biting the heart by mistake. The panel slides off very easily. There's a reason that lithium ion batteries aren't usually easily accessible. I'm not risking my dog discovering a battery and wire in the heart. Even with appropriate supervision, accidents happen. I don't even understand why I would need to access the battery when it's rechargeable. I don't recommend purchasing this product for that reason alone.
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Reviewed in the United States on July 17, 2022
★★★★★ 4
Works great!
It works great, don't have to buy batteries.
07/-8/25
Marilyn A.
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Reviewed in the United States on July 9, 2025
★★★★★ 5
bottle baby kittens preferred this heartbeat to having warm snuggle safe heat disk
Ok... when I got this I thought.. "are you kidding me?" they are going to like this.
Joke was on me. They love it. It is rhythmic and it is strong enough that it pulsates the area so all of them can feel it. They consistently would lay on top of the heart rather than in the area of their "crib" where the heat was.. and this was when they were still only a few days old! It works.
So this is what you want to know.
It beats continuously for about 4-5 days without needing a recharge.
Recharge is fast and only takes a couple hours
The button is slightly "inside" the unit so when it is placed upside down there is no way that it can accidentally be turned off. Which is great. If the button were on the upside in the pet space then a foot or body part of the little animal could turn it off inadvertantly. Design to put the button slightly inset is extremely important design feature - makes this excellent. When I turn it on I know it will be going as long as the battery still has charge. .
I don't think it is waterproof so I put it under the pee pad that I had under the blanket bedding and it was still easily heard AND felt. If not using a pee pad I would definitely put it in a small ziplock bag to protect from any leaked urine or liquids to keep it safe.
When the kittens were done eating they would gravitate towards the heart beat... (with their eyes open or when they were still closed after birth it was the same response). This little gadget was a total lifesaver as it really calmed the kittens in ways I would not be able to.
These pictures are of the trio that LOVED this.... Meet Zen (tabby M with white feet), Sugar (tuxedo F with white feet) and Rocket (ginger - M). All cisgender. they were about 3 days old when I got them to foster.. photos from arrival until about 3 weeks of age. Photo with q-tip is to give relative size on arrival. They will be up for adoption in Tustin CA in another 2-3 weeks time.
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Reviewed in the United States on September 17, 2023
★★★★★ 5
Fantastic!
The heart that came with Snuggle Puppy was a pain to replace the battery. This is the only rechargeable heart I could find searching online. I was a little concerned reading it’s a “frequently returned item.” But happy to say, we will not be returning this! It shipped charged and the heart is still beating non-stop days later! It’s sturdy and fits in the snuggle puppy perfectly. We have a tiny 17 yr old Chihuahua who never chewed anything up so it’s completely safe for her to enjoy. It helps with her anxiety. You can hear the beat in the video—it’s nice and loud and has a bit of a vibration. Our pup has been sleeping next to it every night. Very happy with this purchase. From reading reviews, I trust if we have an issue with it, their customer service will help. For now, it works! I really like that it has time options—can set it for 2 hrs of heart beating or non-stop.
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Reviewed in the United States on September 17, 2024